The protease inhibitor leupeptin prevented multiplication of the human coronavirus strain 299E in cultures of MRC-C cells. The IC50 of leupeptin in plaque reduction tests was 0.4 ฮผg/ml, whereas growth of host cells was unaffected by leupeptin at 50 ฮผg/ml. Inhibition of plaque formation could be prevented by the addition of proteases to the overlay medium. In single-cycle growth experiments, leupeptin reduced virus yield only if added within 2 h of infection, indicating its action on an early stage of virus replication.
year โฐ 1985
issn ๐Ÿ—„ 00221317
volume 66
number 2
page 363-366
citedbycount 13