📄 Sequence of the nucleocapsid gene from murine coronavirus MHV-A59

The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has heen determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector Ml 3 mp8 for direct sequence determination. A sequence of 1817 nu-cleotides, adjacent to the viral poly-A tail, was obtained. It contains a single long open reading frame encoding a protein of mol. wt. 49660, which is enriched in basic residues. © 1983 IRL Press Limited.

keywords 🔗 reading frame (222) 🔗 nucleotide sequence (93) 🔗 nucleocapsid protein (162) 🔗 infected cells (307) 🔗 open reading (215)

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