๐ Quasispecies composition and phylogenetic analysis of feline coronaviruses (FCoVs) in naturally infected cats
Quasispecies composition and tissue distribution of feline coronaviruses (FCo. Vs) were studied in naturally infected cats. The genomic complexity of FCo. Vs was investigated using single-strand conformational polymorphism (SSCP) analysis of N and ORF7b amplicons, and the evolutionary process was investigated by sequence-based phylogenetic analysis. SSCP analysis showed high heterogeneity of the FCoV genome which was correlated with the seriousness of the clinical form. The two genomic regions analysed showed different levels of variation; the N region demonstrated significant heterogeneity as compared to ORF7b. Phylogenetic analysis of the nucleotide sequences showed the clear separation of sequences analysed on the basis of virulence and geographical origin. A maximum likelihood analysis of N and ORF7b data sets showed a situation of strong heterogeneity for the N region. ยฉ 2003 Federation of European Microbiological Societies. Published by Elsevier B. V.
keywords
๐ nucleotide sequence (93)
๐ phylogenetic analysis (188)
๐ feline coronavirus (148)
author
๐ค Battilani, Mara
๐ค Coradin, Tiziana
๐ค Scagliarini, Alessandra
๐ค Ciulli, Sara
๐ค Ostanello, Fabio
๐ค Prosperi, Santino
๐ค Morganti, Luigi
year
โฐ 2003
issn
๐ 09288244
volume
39
number
2
page
141-147
citedbycount
20
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