📄 An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine

The full-length spike (S) gene of porcine respiratory coronavirus (PRCV) was inserted into the genome of human adenovirus type 5 downstream of the early transcription region 3 promoter. The recombinant virus replicated in cultures of the swine testicle ST cell line and directed the synthesis of S antigen with a maximum yield of approximately 26 μg per 106 cells. The antigen was cell-associated except in the late phase of the infection, when a small amount (3-5 μg per 106 cells) was released. The cell-associated antigen consisted of polypeptides of molecular mass 160 kDa and 175 kDa, comigrating with the authentic precursor S′ and the mature S protein of PRCV, respectively. The extra-cellular recombinant antigen corresponded to the 175 kDa mature protein. Some recombinant S protein was exposed on the cell surface and was recognized by neutralization-mediating anti-S monoclonal antibodies. Piglets, inoculated oronasally with the recombinant adenovirus vector developed PRCV-neutralizing serum antibodies and were partially protected against PRCV challenge, demonstrating the potential of live adenovirus as vaccine vector.

keywords 🔗 monoclonal antibodies (131) 🔗 cell surface (110) 🔗 cell line (211) 🔗 porcine respiratory (61) 🔗 respiratory coronavirus (129)

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