📄 Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus

The complete nucleotide sequence of polyprotein gene 1 and the assembled full-length genome sequence are presented for turkey coronavirus (TCoV) isolates 540 and ATCC. The TCoV polyprotein gene encoded two open reading frames (ORFs), which are translated into two products, pp1a and pp1ab, the latter being produced via -1 frameshift translation. TCoV polyprotein pp1a and pp1ab were predicted to be processed to 15 non-structure proteins (nsp2-nsp16), with nsp1 missing. ClustalW analysis revealed 88.99% identity and 96.99% similarity for pp1ab between TCoV and avian infectious bronchitis virus (IBV) at the amino acid level. The whole genome consists of 27,749 nucleotides for 540 and 27,816 nucleotides for ATCC, excluding the poly(A) tail. A total of 13 ORFs were predicted for TCoV. Five subgenomic RNAs were detected from ATCC-infected turkey small intestines by Northern blotting. The whole genome sequence had 86.9% identity between TCoV and IBV, supporting that TCoV is a group 3 coronavirus.

keywords 🔗 reading frames (100) 🔗 reading frame (222) 🔗 nucleotide sequence (93) 🔗 bronchitis virus (233) 🔗 genome sequence (133) 🔗 amino acid (454) 🔗 avian infectious (53) 🔗 infectious bronchitis (235) 🔗 turkey coronavirus (48) 🔗 open reading (215) 🔗 small intestine (43)

download 🔖 [BibTeX]