📄 Development and evaluation of novel real-time reverse transcription-PCR assays with locked nucleic acid probes targeting leader sequences of human-pathogenic coronaviruses

Copyright © 2015, American Society for Microbiology. Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.

keywords 🔗 syndrome coronavirus (1074) 🔗 reverse transcription (205) 🔗 human coronavirus (623) 🔗 real-time reverse (87) 🔗 nucleic acid (139) 🔗 respiratory syndrome (2004) 🔗 leader sequence (32)

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