๐ Respiratory disease associated with bovine coronavirus infection in cattle herds in Southern Italy
Four outbreaks of bovine respiratory disease (BRD) associated with bovine coronavirus (BCoV) infection in Italian cattle herds were reported. In 3 outbreaks, BRD was observed only in 2-3-month-old feedlot calves, whereas in the remaining outbreak, lactating cows, heifers, and calves were simultaneously affected. By using reverse transcription polymerase chain reaction (RT-PCR), BCoV RNA was detected in all outbreaks without evidence of concurrent viral pathogens (i.e., bovine respiratory syncytial virus, bovine herpesvirus type 1, bovine viral diarrhea virus, bovine parainfluenza virus). Common bacteria of cattle were recovered only from 2 outbreaks of BRD: Staphylococcus spp. and Proteus mirabilis (outbreak 1) and Mannheimia haemolytica (outbreak 4). A recently established real-time RT-PCR assay showed that viral RNA loads in nasal secretions ranged between 3.10 x 102 and 7.50 x 107 RNA copies/ฮผl of template. Bovine coronavirus was isolated from respiratory specimens from all outbreaks except outbreak 1, in which real-time RT-PCR found very low viral titers in nasal swabs.
keywords
๐ bovine coronavirus (221)
๐ respiratory syncytial (49)
๐ reverse transcription (205)
๐ polymerase chain (300)
๐ nasal swab (58)
๐ chain reaction (303)
author
๐ค Decaro, Nicola
๐ค Campolo, Marco
๐ค Desario, Costantina
๐ค Cirone, Francesco
๐ค D'Abramo, Maria
๐ค Lorusso, Eleonora
๐ค Greco, Grazia
๐ค Mari, Viviana
๐ค Colaianni, Maria Loredana
๐ค Elia, Gabriella
๐ค Martella, Vito
๐ค Buonavoglia, Canio
year
โฐ 2008
issn
๐ 10406387
volume
20
number
1
page
28-32
citedbycount
34
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