📄 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay for SARS-associated Coronavirus

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

keywords 🔗 severe acute (1373) 🔗 transcription-polymerase chain (58) 🔗 reverse transcription (205) 🔗 respiratory syndrome-associated (90) 🔗 polymerase chain (300) 🔗 real-time reverse (87) 🔗 clinical specimens (44) 🔗 reverse transcription-polymerase (55) 🔗 respiratory syndrome (2004) 🔗 acute respiratory (1734) 🔗 syndrome-associated coronavirus (88) 🔗 chain reaction (303)

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