📄 The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: Expression and functional characterization

The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis. © 2005 Federation of European Biochemical Societies. Published by Elsevier B. V.

keywords 🔗 reading frame (222) 🔗 endoplasmic reticulum (78) 🔗 open reading (215)

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