๐ Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in Australia
Objective Compare real-time reverse transcription polymerase chain reaction (qRT-PCR), a commercially available enzyme-linked immunosorbent assay (ELISA) and lateral flow immunochromatography assay (LAT) for the detection of rotavirus and coronavirus in faecal samples collected from diarrhoeic calves. Design Prospective survey. Method Samples were tested at two separate facilities using a commercial ELISA and an in-house qRT-PCR. Simple logistic regression was performed to examine the relationship between the two tests. A subset of samples was screened using qRT-PCR, ELISA and a commercial LAT dipstick (132 faecal samples were tested for coronavirus and 122 samples for rotavirus). Results Of the 586 samples tested, 131 (22.39%) and 468 (79.86%) were positive for coronavirus and group A rotavirus, respectively, using qRT-PCR. The number of samples positive on ELISA for coronavirus and rotavirus was 73 (12.46%) and 225 (38.40%), respectively. Using LAT, 30 (22.73%) and 43 (35.35%) samples were positive for coronavirus and rotavirus, respectively. Simple linear regression revealed a statistically significant (P < 0.05) but weak (r 2=-0.07 and -0.40) correlation between the rotavirus/coronavirus qRT-PCR and ELISA, respectively. There was also poor agreement between the LAT and qRT-PCR assays. Conclusion The sensitivity and specificity of the commercial ELISA and LAT assays evaluated in this study were low compared with qRT-PCR. The low positive and negative predictive values of the assays suggests that they were of limited diagnostic benefit in the population sampled. Australian Veterinary Journal ยฉ 2012 Australian Veterinary Association.
keywords
๐ samples collected (74)
๐ reverse transcription (205)
๐ statistically significant (37)
๐ polymerase chain (300)
๐ real-time reverse (87)
๐ faecal samples (44)
๐ enzyme-linked immunosorbent (105)
๐ logistic regression (35)
๐ immunosorbent assay (114)
๐ chain reaction (303)
author
๐ค Izzo, M. M.
๐ค Kirkland, P. D.
๐ค Gu, X.
๐ค Lele, Y.
๐ค Gunn, A. A.
๐ค House, J. K.
year
โฐ 2012
issn
๐ 00050423 17510813
volume
90
number
4
page
122-129
citedbycount
16
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