📄 SARS-associated Coronavirus Replication in Cell Lines

Given the potential for laboratory-associated severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also used as a confirmatory test. The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects.

keywords 🔗 severe acute (1373) 🔗 cytopathic effect (91) 🔗 cell lines (125) 🔗 respiratory syndrome-associated (90) 🔗 polymerase chain (300) 🔗 respiratory syndrome (2004) 🔗 acute respiratory (1734) 🔗 cell line (211) 🔗 syndrome-associated coronavirus (88) 🔗 chain reaction (303) 🔗 previously reported (79) 🔗 viral replication (258)

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