📄 Amino acids 15-28 in the ectodomain of SARS coronavirus 3a protein induces neutralizing antibodies

A synthetic peptide corresponding to amino acids (aa) 15-28 of the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein was used to raise polyclonal antibodies in rabbits. This anti-3a N-terminal antibody could detect 3a protein in infected cells, as did an anti-3a C-terminal antibody previously described. The latter targeted the C-terminal cytoplasmic domain of 3a (aa 134-274). The anti-3a N-terminal antibody could detect intracellular 3a as well as 3a expressed on the cell surface. Interestingly, only the anti-3a N-terminal antibody can inhibit SARS-CoV propagation in Vero E6 culture although the binding affinity of the anti-3a N-terminal antibody was lower than the anti-3a C-terminal antibody. © 2006 Federation of European Biochemical Societies.

keywords 🔗 severe acute (1373) 🔗 syndrome coronavirus (1074) 🔗 previously described (38) 🔗 amino acid (454) 🔗 cell surface (110) 🔗 infected cells (307) 🔗 respiratory syndrome (2004) 🔗 acute respiratory (1734) 🔗 amino acids (205)

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