📄 The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop fold

The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is regulated by specific viral genome sequences within 5′- and 3′-untranslated regions (5′-UTR and 3′-UTR). Here we report the solution structure of 5′-UTR derived stem-loop 2 (SL2) of SARS-CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5-bp stem and adopts a canonical CUYG tetraloop fold with the 3′ nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed. © 2011 Federation of European Biochemical Societies. Published by Elsevier B. V.

keywords 🔗 severe acute (1373) 🔗 hepatitis virus (437) 🔗 genome sequence (133) 🔗 mouse hepatitis (371) 🔗 viral genome (96) 🔗 respiratory syndrome (2004) 🔗 highly conserved (80) 🔗 acute respiratory (1734) 🔗 genome sequences (60)

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