We report a familial cluster of 2019 novel coronavirus disease (COVID-19) to evidence that a potential transmission of the COVID-19 during the incubation period. The first patient in this familial cluster was identified in presymptomatic period, as a close contact of a confirmed patient. Five family members had close contact with the first patient during his incubation period, four of them were confirmed to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in the subsequent sampling test.