📄 Detection of severe acute respiratory syndrome coronavirus in stool specimens by commercially available real-time reverse transcriptase PCR assays

Three commercially available real-time reverse transcriptase PCR assays (the Artus Real. Art HPA coronavirus Light. Cycler, the Artus Real. Art HPA coronavirus Rotor-Gene, and the Era. Gen severe acute respiratory syndrome coronavirus POL assay) and three RNA extraction methodologies were evaluated for the detection of severe acute respiratory syndrome coronavirus RNA from 91 stool specimens. The assays' sensitivities were highest (58% to 75%) for specimens obtained 8 to 21 days after symptom onset The assays were less sensitive when specimens were obtained less than 8 days or more than 21 days after the onset of symptoms. All assays were 100% specific. Copyright © 2006, American Society for Microbiology.

keywords 🔗 severe acute (1373) 🔗 syndrome coronavirus (1074) 🔗 symptom onset (46) 🔗 reverse transcriptase (87) 🔗 real-time reverse (87) 🔗 respiratory syndrome (2004) 🔗 acute respiratory (1734)

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