📄 Detection of novel ferret coronaviruses and evidence of recombination among ferret coronaviruses

© 2016, Springer Science+Business Media New York. In an epidemiological study of ferret coronaviruses (FRCo. Vs), novel FRCoV strains (Saitama-1 and Aichi-1) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequence analysis of partial RNA-dependent RNA polymerase (Rd. Rp) genes. Phylogenetic analysis indicated that these strains belonged to different clusters from other FRCoV strains. Next, the nucleotide sequence of the 3′-terminal region of Saitama-1 (8271 bases) strain was determined and compared with those of the other FRCo. Vs, indicating that the Saitama-1 strain differed from the previously reported MSU-1 and MSU-2 strains in the regions encoding spike (S) protein, nucleocapsid, and open reading frame 7b. Furthermore, the results of Sim. Plot analysis indicated that FRCoV (MSU-2 strain) emerged via a recombination event of S protein between the MSU-1 and Saitama-1 strains. This mechanism is similar to that responsible for the emergence of type II feline coronavirus. This information will be useful for understanding the pathogenesis of FRCoV in ferrets.

keywords 🔗 sequence analysis (118) 🔗 reading frame (222) 🔗 nucleotide sequence (93) 🔗 transcription-polymerase chain (58) 🔗 reverse transcription (205) 🔗 polymerase chain (300) 🔗 reverse transcription-polymerase (55) 🔗 feline coronavirus (148) 🔗 open reading (215) 🔗 chain reaction (303) 🔗 previously reported (79)

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