📄 Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridization

A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridization. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single‐stranded RNA probe. Nucleic acid hybridization is shown to be a sensitive technique for the diagnosis of HCV 229E infections. Copyright © 1989 Wiley‐Liss, Inc., A Wiley Company

keywords 🔗 human coronavirus (623)

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