📄 Detection and molecular characterization of calf diarrhoea bovine coronaviruses circulating in South Korea during 2004-2005

Although the widespread occurrence of calf diarrhoea (CD) bovine coronavirus (BCoV) infections have been reported in most cattle producing countries, only the genetic differences in the BCo. Vs from American and Canadian isolates and/or strains have been identified and compared. Hence, it is unclear if the BCo. Vs circulating in the other countries have distinct genetic characteristics. The aim of this study was to determine the prevalence and genetic diversity of CD BCo. Vs based on the deduced amino acid (aa) sequences of the spike (S) and haemagglutinin/esterase (HE) proteins in South Korea. RT-PCR and nested PCR using the primer pairs specific to the nucleocapsid gene, BCo. Vs detected the BCo. Vs in 56 (15.6%) of 359 diarrhoeic faecal samples. Phylogenetic analysis of the entire S gene indicated that 10 Korean CD BCoV strains clustered with other Korean BCoV strains with different clinical forms but were different from the American and Canadian BCoV strains. Moreover, the phylogenetic data of the aa sequences of the HE gene revealed all the Korean CD strains to be distinct from the other Korean BCoV strains with different clinical forms. These results suggest that the Korean BCo. Vs cause endemic infections in diarrhoeic calves in Jeonnam province and have taken a different evolutionary pathway from the BCo. Vs in other countries. Moreover, the different BCoV strains are circulating in the different clinical forms in South Korea. These results also suggest that vaccines against the BCo. Vs can be developed with each Korean BCoV in different clinical forms.

keywords 🔗 bovine coronavirus (221) 🔗 amino acid (454) 🔗 faecal samples (44) 🔗 results suggest (206)

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