📄 Isolation of middle east respiratory syndrome coronavirus from a patient of the 2015 Korean outbreak

©2016 The Korean Academy of Medical Sciences. During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.

keywords 🔗 syndrome coronavirus (1074) 🔗 genome sequence (133) 🔗 respiratory syndrome (2004) 🔗 cell culture (240) 🔗 electron microscopy (149)

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