📄 Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells

The nucleotide sequence of the S peplomer gene of bovine coronavirus (BCV) has been determined. A single open reading frame of 4089 nucleotides encodes a polypeptide of 150K with 20 potential sites for addition of N-linked oligosaccharides. Expression of the cloned BCV S gene by a recombinant Autographa californica nuclear polyhedrosis virus resulted in production of a 180K glycosylated polypeptide which was transported to the surface of the cell. Comparison of the BCV S gene with the analogous genes of murine hepatitis viruses shows that the BCV S polypeptide contains a unique domain of 138 amino acids not present in murine hepatitis virus strain JHM, but which has a partially homologous counterpart in strain A59. This domain accounts for most of the differences in size of the S gene products of these coronaviruses.

keywords 🔗 bovine coronavirus (221) 🔗 reading frame (222) 🔗 nucleotide sequence (93) 🔗 hepatitis virus (437) 🔗 amino acid (454) 🔗 murine hepatitis (71) 🔗 amino acids (205) 🔗 virus strain (138) 🔗 open reading (215)

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