📄 High level transient expression of the murine coronavirus haemagglutinin-esterase

We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemadsorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.

keywords 🔗 significantly higher (104) 🔗 infected cells (307) 🔗 murine coronavirus (140)

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