A sensitive and highly specific enzyme-linked Immunosorbent assay (ELISA) system for detection of bovine coronavirus was developed using monoclonal antibodies, which showed both neutralization and hemagglutination inhibition activities. A monoclonal antibody was coated onto microplates for capturing antigen and a mixture of two monoclonal antibodies served as detecting antibodies. Using this assay, 40 of 202 fecal specimens from 29 herds were shown to be positive for viral antigen. From 10 ELISA positive specimens from 5 herds, the virus was isolated after several passages on human rectal adenocarcinoma (HRT-18) cell culture. © 1993, JAPANESE SOCIETY OF VETERINARY SCIENCE.
year ⏰ 1993
issn 🗄 13477439 09167250
volume 55
number 5
page 771-774
citedbycount 5
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