π The synergistic interaction of interferon types I and II leads to marked reduction in severe acute respiratory syndrome-associated coronavirus replication and increase in the expression of mRNAs for interferon-induced proteins
Interferon (IFN)-Ξ±, -Ξ² and -Ξ³ have been shown to be only marginally effective against severe acute respiratory syndrome coronavirus (SARS-CoV) replication in Vero cell lines. We investigated the combination of type I IFNs (IFN-Ξ± or -Ξ²) and IFN-Ξ³ for antiviral activity and found that such combinations synergistically inhibited SARS-CoV replication in Vero cells, using yield reduction assay and the isobologram and combination index methods of Chou and Talalay for evaluation. The highly synergistic anti-SARS-CoV action of type I IFNs and IFN-Ξ³ parallels the marked increase in 2β²-5β²-oligoadenylate synthetase and p56 mRNAs following exposure in Vero cells to either IFN-Ξ± or -Ξ² and IFN-Ξ³ compared with the transcriptional levels obtained after stimulation with either IFN alone. These results demonstrate that SARS-CoV, although only moderately sensitive to the antiviral action of the individual types of IFN, is highly sensitive to a combination of type I and II IFNs, which suggests that such combinations may have potential in the treatment of SARS-CoV infections. Copyright Β© 2007 S. Karger AG.
keywords
π severe acute (1373)
π syndrome coronavirus (1074)
π cell lines (125)
π respiratory syndrome (2004)
π acute respiratory (1734)
π cell line (211)
author
π€ Scagnolari, Carolina
π€ Trombetti, Simona
π€ Alberelli, Alessia
π€ Cicetti, Simona
π€ Bellarosa, Daniela
π€ Longo, Roberta
π€ SpanΓ², Alberto
π€ Riva, Elisabetta
π€ Clementi, Massimo
π€ Antonelli, Guido
year
β° 2007
journal
π Intervirology
issn
π 03005526
volume
50
number
2
page
156-160
citedbycount
13
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