📄 Diagnosis of Severe Acute Respiratory Syndrome (SARS) by Detection of SARS Coronavirus Nucleocapsid Antibodies in An Antigen-Capturing Enzyme-Linked Immunosorbent Assay

Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (ELISA). Antinucleocapsid protein antibodies could be detected in 68.4% of probable SARS patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. No false-positive results were observed in 20 non-SARS fever patients, 24 non-SARS respiratory illness patients, and 20 health care workers. Among 940 other non-SARS clinical serum samples, only 1 was found to be weakly positive. This method provides a new, sensitive, and specific approach for SARS diagnosis.

keywords 🔗 severe acute (1373) 🔗 serum samples (106) 🔗 nucleocapsid protein (162) 🔗 enzyme-linked immunosorbent (105) 🔗 respiratory syndrome (2004) 🔗 immunosorbent assay (114) 🔗 acute respiratory (1734) 🔗 health care (123)

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