📄 Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels

Copyright © 2015, American Society for Microbiology. We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.

keywords 🔗 syndrome coronavirus (1074) 🔗 monoclonal antibodies (131) 🔗 reading frame (222) 🔗 dromedary camels (70) 🔗 nucleocapsid protein (162) 🔗 reverse transcriptase (87) 🔗 real-time reverse (87) 🔗 respiratory syndrome (2004) 🔗 nasal swab (58) 🔗 results suggest (206) 🔗 open reading (215)

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