📄 Role of IFN-γ responsiveness in CD8 T cell-mediated viral clearance and demyelination in coronavirus-infected mice

Immunocompetent, but not RAG1-/- mice infected with MHV-JHM develop demyelination. Transferred CD8 T cell-enriched splenocytes reconstitute demyelination, and this ability is dependent on donor IFN-γ. We used IFN-γR1-/- mice to examine the target of IFN-γ in CD8 T cell-mediated demyelination. In IFN-γR1-/-RAG1-/- recipients, demyelination is decreased, but not eliminated, while viral titers are significantly increased when compared to IFN-γR1+/+RAG1-/- recipients. IFN-γR1-/- CD8 T cells retain virus-specific effector function regardless of IFN-γR1 expression. Although IFN-γR1 responsiveness is critical for maximal demyelination, increased levels of infectious virus coupled with adoptive transfer of CD8 T cells may result in myelin destruction independent of IFN-γR1 expression.

keywords 🔗 infectious virus (88) 🔗 adoptive transfer (14)

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