📄 Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction
An RT-PCR-hybridization was developed that amplified genetic material from the M protein gene of HCoV-229E and HCoV-OC43. The analytic sensitivity of these original primers were compared with primers defined in the N gene and described previously. The results show that 0.05 TCID50 of HCoV-229E and 0.01 TCID50 of HCoV-OC43 can be detected by this molecular method using the original method. Detection of HCoV-229E and HCoV-OC43 in clinical specimens is possible using this method: 348 respiratory specimens (202 sputum and 146 nasal aspirates) were tested with this RT-PCR-hybridization and 12 human coronavirus are detected (3%). The method could provide a useful tool for demonstrating the role of human coronavirus in infections of the respiratory tract. © 2001 Elsevier Science B. V.
author
👤 Vabret, Astrid
👤 Mouthon, Franck
👤 Mourez, Thomas
👤 Gouarin, Stephanie
👤 Petitjean, Joëlle
👤 Freymuth, François
year
⏰ 2001
journal
📚 Journal of Virological Methods
issn
🗄 01660934
volume
97
number
1-2
page
59-66
citedbycount
61
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