๐ Experimental infection of conventional nursing pigs and their dams with Porcine deltacoronavirus
ยฉ 2016, Porcine deltacoronavirus (PDCoV) is a newly identified virus that has been detected in swine herds of North America associated with enteric disease. The aim of this study was to demonstrate the pathogenicity, course of infection, virus kinetics, and aerosol transmission of PDCoV using 87 conventional piglets and their 9 dams, including aerosol and contact controls to emulate field conditions. Piglets 2โ4 days of age and their dams were administered an oronasal PDCoV inoculum with a quantitative real-time reverse transcription (qRT)-PCR quantification cycle (Cq) value of 22 that was generated from a field sample having 100% nucleotide identity to USA/Illinois121/2014 determined by metagenomic sequencing and testing negative for other enteric disease agents using standard assays. Serial samples of blood, serum, oral fluids, nasal and fecal swabs, and tissues from sequential autopsy, conducted daily on days 1โ8 and regular intervals thereafter, were collected throughout the 42-day study for qRT-PCR, histopathology, and immunohistochemistry. Diarrhea developed in all inoculated and contact control pigs, including dams, by 2 days post-inoculation (dpi) and in aerosol control pigs and dams by 3โ4 dpi, with resolution occurring by 12 dpi. Mild to severe atrophic enteritis with PDCoV antigen staining was observed in the small intestine of affected piglets from 2 to 8 dpi. Mesenteric lymph node and small intestine were the primary sites of antigen detection by immunohistochemistry, and virus RNA was detected in these tissues to the end of the study. Virus RNA was detectable in piglet fecal swabs to 21 dpi, and dams to 14โ35 dpi.
keywords
๐ reverse transcription (205)
๐ lymph node (56)
๐ real-time reverse (87)
๐ small intestine (43)
author
๐ค Vitosh-Sillman, Sarah
๐ค Loy, John Dustin
๐ค Brodersen, Bruce
๐ค Kelling, Clayton
๐ค Doster, Alan
๐ค Topliff, Christina
๐ค Nelson, Eric
๐ค Bai, Jianfa
๐ค Schirtzinger, Erin
๐ค Poulsen, Elizabeth
๐ค Meadors, Barbara
๐ค Anderson, Joseph
๐ค Hause, Benjamin
๐ค Anderson, Gary
๐ค Hesse, Richard
year
โฐ 2016
issn
๐ 19434936 10406387
volume
28
number
5
page
486-497
citedbycount
6
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