📄 Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system

The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192). Copyright © 2005, American Society for Microbiology.

keywords 🔗 severe acute (1373) 🔗 infectious disease (312) 🔗 respiratory syndrome-associated (90) 🔗 important role (140) 🔗 antibody responses (58) 🔗 respiratory syndrome (2004) 🔗 acute respiratory (1734) 🔗 syndrome-associated coronavirus (88)

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