📄 Coronavirus JHM: Characterization of intracellular viral RNA

After infection of Sac (-) cells with the murine coronavirus JHM the synthesis of seven major and two minor RNA species was induced. These RNAs were polyadenylated and single-stranded. Their mol. wt. were estimated by electrophoresis in agarose gels containing methylmercury hydroxide. The values for the major species were 6.67 x 106 for RNA of genome size (RNA 1), 3.42 x 106 for RNA 2, 2.76 x 106 for RNA 3, 1.35 x 106 for RNA 4, 1.19 x 106 for RNA 5, 0.93 x 106 for RNA 6 and 0.62 x 106 for RNA 7. The minor species have a size of 4.7 x 106 (RNA a) and 1.5 x 106 (RNA b). The same number of species were found by electrophoresis after denaturation with glyoxal-dimethyl sulphoxide. No gross difference in number of RNAs and the amount of each species was found between total cytoplasmic RNA, polyadenylated cytoplasmic RNA and RNA extracted from pelleted polysomes.

keywords 🔗 murine coronavirus (140)

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