📄 False-positive results in a recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by western blotting with recombinant SARS-CoV spike polypeptide

Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.

keywords 🔗 severe acute (1373) 🔗 serum samples (106) 🔗 nucleocapsid protein (162) 🔗 human coronavirus (623) 🔗 enzyme-linked immunosorbent (105) 🔗 respiratory syndrome (2004) 🔗 immunosorbent assay (114) 🔗 acute respiratory (1734) 🔗 samples obtained (25)

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