📄 Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex

To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap Ado. Met-dependent (nucleoside-2′- O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress. © 2011 International Union of Crystallography

keywords 🔗 highly pathogenic (100) 🔗 fatality rate (123) 🔗 human coronavirus (623) 🔗 crystal structure (114)

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