๐ Severe acute respiratory syndrome-associated coronavirus vaccines formulated with delta inulin adjuvants provide enhanced protection while ameliorating lung eosinophilic immunopathology
ยฉ 2015, American Society for Microbiology. Although the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) epidemic was controlled by nonvaccine measures, coronaviruses remain a major threat to human health. The design of optimal coronavirus vaccines therefore remains a priority. Such vaccines present major challenges: coronavirus immunity often wanes rapidly, individuals needing to be protected include the elderly, and vaccines may exacerbate rather than prevent coronavirus lung immunopathology. To address these issues, we compared in a murine model a range of recombinant spike protein or inactivated whole-virus vaccine candidates alone or adjuvanted with either alum, CpG, or Advax, a new delta inulin-based polysaccharide adjuvant. While all vaccines protected against lethal infection, addition of adjuvant significantly increased serum neutralizing-antibody titers and reduced lung virus titers on day 3 postchallenge. Whereas unadjuvanted or alum-formulated vaccines were associated with significantly increased lung eosinophilic immunopathology on day 6 postchallenge, this was not seen in mice immunized with vaccines formulated with delta inulin adjuvant. Protection against eosinophilic immunopathology by vaccines containing delta inulin adjuvants correlated better with enhanced T-cell gamma interferon (IFN-ฮณ) recall responses rather than reduced interleukin-4 (IL-4) responses, suggesting that immunopathology predominantly reflects an inadequate vaccine-induced Th1 response. This study highlights the critical importance for development of effective and safe coronavirus vaccines of selection of adjuvants based on the ability to induce durable IFN-ฮณ responses.
keywords
๐ severe acute (1373)
๐ mice immunized (25)
๐ spike protein (353)
๐ respiratory syndrome-associated (90)
๐ respiratory syndrome (2004)
๐ acute respiratory (1734)
๐ syndrome-associated coronavirus (88)
author
๐ค Honda-Okubo, Yoshikazu
๐ค Barnard, Dale
๐ค Ong, Chun Hao
๐ค Peng, Bi Hung
๐ค Tseng, Chien Te Kent
๐ค Petrovsky, Nikolai
year
โฐ 2015
journal
๐ Journal of Virology
issn
๐ 10985514 0022538X
volume
89
number
6
page
2995-3007
citedbycount
31
download
๐ [BibTeX]