📄 Transmissible gastroenteritis coronavirus RNA-dependent RNA polymerase and nonstructural proteins 2, 3, and 8 are incorporated into viral particles

Coronavirus replication and transcription are processes mediated by a protein complex, with the RNA-dependent RNA polymerase (Rd. Rp) as a main component. Proteomic analysis of highly purified transmissible gastroenteritis virus showed the Rd. Rp to be a component of the viral particles. This finding was confirmed by Western blotting, immunofluorescence, and immunoelectron microscopy analyses. Interestingly, the replicase nonstructural proteins 2, 3, and 8 colocalized with the Rd. Rp in the viral factories and were also incorporated into the virions. © 2012, American Society for Microbiology.

keywords 🔗 immunoelectron microscopy (18) 🔗 nonstructural proteins (57) 🔗 viral particles (45) 🔗 virus replication (219) 🔗 gastroenteritis virus (188) 🔗 structural proteins (197) 🔗 electron microscopy (149) 🔗 transmissible gastroenteritis (226)

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