📄 Molecular characterization of a novel coronavirus associated with epizootic catarrhal enteritis (ECE) in ferrets

A novel coronavirus, designated as ferret enteric coronavirus (FECV), was identified in feces of domestic ferrets clinically diagnosed with epizootic catarrhal enteritis (ECE). Initially, partial sequences of the polymerase, spike, membrane protein, and nucleocapsid genes were generated using coronavirus consensus PCR assays. Subsequently, the complete sequences of the nucleocapsid gene and the last two open reading frames at the 3′ terminus of the FECV genome were obtained. Phylogenetic analyses based on predicted partial amino acid sequences of the polymerase, spike, and membrane proteins, and full sequence of the nucleocapsid protein showed that FECV is genetically most closely related to group 1 coronaviruses. FECV is more similar to feline coronavirus, porcine transmissible gastroenteritis virus, and canine coronavirus than to porcine epidemic diarrhea virus and human coronavirus 229E. Molecular data presented in this study provide the first genetic evidence for a new coronavirus associated with clinical cases of ECE. © 2006 Elsevier Inc.

keywords 🔗 reading frames (100) 🔗 closely related (222) 🔗 reading frame (222) 🔗 acid sequence (108) 🔗 amino acid (454) 🔗 acid sequences (44) 🔗 enteric coronavirus (111) 🔗 nucleocapsid protein (162) 🔗 human coronavirus (623) 🔗 novel coronavirus (684) 🔗 gastroenteritis virus (188) 🔗 canine coronavirus (102) 🔗 membrane protein (93) 🔗 feline coronavirus (148) 🔗 open reading (215) 🔗 transmissible gastroenteritis (226)

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