๐ Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library
A 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (โผ66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S471-503, a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S471-503 peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion. ยฉ 2005 American Chemical Society.
keywords
๐ severe acute (1373)
๐ host cell (262)
๐ converting enzyme (162)
๐ structural proteins (197)
๐ receptor binding (86)
๐ respiratory syndrome (2004)
๐ angiotensin-converting enzyme (112)
๐ acute respiratory (1734)
author
๐ค Hu, Hongbo
๐ค Li, Li
๐ค Kao, Richard Y.
๐ค Kou, Binbin
๐ค Wang, Zhanguo
๐ค Zhang, Liang
๐ค Zhang, Huiyuan
๐ค Hao, Zhiyong
๐ค Tsui, Wayne H.
๐ค Ni, Anping
๐ค Cui, Lianxian
๐ค Fan, Baoxing
๐ค Guo, Feng
๐ค Rao, Shuan
๐ค Jiang, Chengyu
๐ค Li, Qian
๐ค Sun, Manji
๐ค He, Wei
๐ค Liu, Gang
year
โฐ 2005
issn
๐ 15204766
volume
7
number
5
page
648-656
citedbycount
19
download
๐ [BibTeX]