📄 A single point mutation creating a furin cleavage site in the spike protein renders porcine epidemic diarrhea coronavirus trypsin independent for cell entry and fusion

© 2015, American Society for Microbiology. The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of the putative fusion peptide (PEDV-S^FCS). PEDV-S^FCS exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independently of trypsin, though to low titer.

keywords 🔗 cell-cell fusion (34) 🔗 cleavage site (85) 🔗 amino acid (454) 🔗 cell culture (240) 🔗 membrane fusion (105)

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