๐ Receptor usage and cell entry of bat coronavirus HKU4 provide insight into bat-to-human transmission of MERS coronavirus
Middle East respiratory syndrome coronavirus (MERS-CoV) currently spreads in humans and causes โผ36% fatality in infected patients. Believed to have originated from bats, MERS-CoV is genetically related to bat coronaviruses HKU4 and HKU5. To understand how bat coronaviruses transmit to humans, we investigated the receptor usage and cell entry activity of the virus-surface spike proteins of HKU4 and HKU5. We found that dipeptidyl peptidase 4 (DPP4), the receptor for MERS-CoV, is also the receptor for HKU4, but not HKU5. Despite sharing a common receptor, MERS-CoV and HKU4 spikes demonstrated functional differences. First, whereas MERS-CoV prefers human DPP4 over bat DPP4 as its receptor, HKU4 shows the opposite trend. Second, in the absence of exogenous proteases, both MERS-CoV and HKU4 spikes mediate pseudovirus entry into bat cells, whereas only MERS-CoV spike, but not HKU4 spike, mediates pseudovirus entry into human cells. Thus, MERS-CoV, but not HKU4, has adapted to use human DPP4 and human cellular proteases for efficient human cell entry, contributing to the enhanced pathogenesis of MERS-CoV in humans. These results establish DPP4 as a functional receptor for HKU4 and host cellular proteases as a host range determinant for HKU4. They also suggest that DPP4-recognizing bat coronaviruses threaten human health because of their spikes' capability to adapt to human cells for cross-species transmissions.
keywords
๐ syndrome coronavirus (1074)
๐ spike protein (353)
๐ host cell (262)
๐ dipeptidyl peptidase (47)
๐ respiratory syndrome (2004)
author
๐ค Yang, Yang
๐ค Du, Lanying
๐ค Liu, Chang
๐ค Wang, Lili
๐ค Ma, Cuiqing
๐ค Tang, Jian
๐ค Baric, Ralph S.
๐ค Jiang, Shibo
๐ค Li, Fang
year
โฐ 2014
issn
๐ 10916490 00278424
volume
111
number
34
page
12516-12521
citedbycount
103
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