🔗 Reverse genetics

Articles (49)

• Engineering a replication-competent, propagation-defective middle east respiratory syndrome coronavirus as a vaccine candidate

  👤 Almazán, Fernando 👤 Dediego, Marta L. 👤 Sola, Isabel 👤 Zuñiga, Sonia 👤 Nieto-Torres, Jose L. 👤 Marquez-Jurado, Silvia 👤 Andrés, German 👤 Enjuanes, Luis ⏰ 2013 📚 mBio

• Pathogenesis of murine coronavirus in the central nervous system

  👤 Bender, Susan J. 👤 Weiss, Susan R. ⏰ 2010 📚 Journal of Neuroimmune Pharmacology

• Identification of a Noncanonically Transcribed Subgenomic mRNA of Infectious Bronchitis Virus and Other Gammacoronaviruses

  👤 Bentley, Kirsten 👤 Keep, Sarah May 👤 Armesto, Maria 👤 Britton, Paul ⏰ 2013 📚 Journal of Virology

• Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes

  👤 Bouvet, Mickaël 👤 Lugari, Adrien 👤 Posthuma, Clara C. 👤 Zevenhoven, Jessika C. 👤 Bernard, Stéphanie 👤 Betzi, Stéphane 👤 Imbert, Isabelle 👤 Canard, Bruno 👤 Guillemot, Jean Claude 👤 Lécine, Patrick 👤 Pfefferle, Susanne 👤 Drosten, Christian 👤 Snijder, Eric J. 👤 Decroly, Etienne 👤 Morelli, Xavier ⏰ 2014 📚 Journal of Biological Chemistry

• Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection

  👤 Britton, Paul 👤 Evans, Sharon 👤 Dove, Brian 👤 Davies, Marc 👤 Casais, Rosa 👤 Cavanagh, Dave ⏰ 2005 📚 Journal of Virological Methods

• Structural and biological basis of CTL escape in coronavirus-infected mice

  👤 Butler, Noah S. 👤 Theodossis, Alex 👤 Webb, Andrew I. 👤 Dunstone, Michelle A. 👤 Nastovska, Roza 👤 Ramarathinam, Sri Harsha 👤 Rossjohn, Jamie 👤 Purcell, Anthony W. 👤 Perlman, Stanley ⏰ 2008 📚 Journal of Immunology

• Gene 5 of the avian coronavirus Infectious bronchitis virus is not essential for replication

  👤 Casais, Rosa 👤 Davies, Marc 👤 Cavanagh, David 👤 Britton, Paul ⏰ 2005 📚 Journal of Virology

• MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity

  👤 Chu, Daniel K.W. 👤 Hui, Kenrie P.Y. 👤 Perera, Ranawaka A.P.M. 👤 Miguel, Eve 👤 Niemeyer, Daniela 👤 Zhao, Jincun 👤 Channappanavar, Rudragouda 👤 Dudas, Gytis 👤 Oladipo, Jamiu O. 👤 Traoré, Amadou 👤 Fassi-Fihri, Ouafaa 👤 Ali, Abraham 👤 Demissié, Getnet F. 👤 Muth, Doreen 👤 Chan, Michael C.W. 👤 Nicholls, John M. 👤 Meyerholz, David K. 👤 Kuranga, Sulyman A. 👤 Mamo, Gezahegne 👤 Zhou, Ziqi 👤 So, Ray T.Y. 👤 Hemida, Maged G. 👤 Webby, Richard J. 👤 Roger, Francois 👤 Rambaut, Andrew 👤 Poon, Leo L.M. 👤 Perlman, Stanley 👤 Drosten, Christian 👤 Chevalier, Veronique 👤 Peiris, Malik ⏰ 2018 📚 Proceedings of the National Academy of Sciences of the United States of America

• Mutation in murine coronavirus replication protein nsp4 alters assembly of double membrane vesicles

  👤 Clementz, Mark A. 👤 Kanjanahaluethai, Amornrat 👤 O'Brien, Timothy E. 👤 Baker, Susan C. ⏰ 2008 📚 Virology

• Analysis of coronavirus temperature-sensitive mutants reveals an interplay between the macrodomain and Papain-like protease impacting replication and pathogenesis

  👤 Deng, Xufang 👤 Mettelman, Robert C. 👤 O'Brien, Amornrat 👤 Thompson, John A. 👤 O'Brien, Timothy E. 👤 Baker, Susan C. ⏰ 2019 📚 Journal of Virology

• Coronaviruses resistant to a 3C-like protease inhibitor are attenuated for replication and pathogenesis, revealing a low genetic barrier but high fitness cost of resistance

  👤 Deng, Xufang 👤 St John, Sarah E. 👤 Osswald, Heather L. 👤 O'Brien, Amornrat 👤 Banach, Bridget S. 👤 Sleeman, Katrina 👤 Ghosh, Arun K. 👤 Mesecar, Andrew D. 👤 Baker, Susan C. ⏰ 2014 📚 Journal of Virology

• Systematic assembly of a full-length infectious clone of human coronavirus NL63

  👤 Donaldson, Eric F. 👤 Yount, Boyd 👤 Sims, Amy C. 👤 Burkett, Susan 👤 Pickles, Raymond J. 👤 Baric, Ralph S. ⏰ 2008 📚 Journal of Virology

• Reverse genetics for type I feline coronavirus field isolate to study the molecular pathogenesis of feline infectious peritonitis

  👤 Ehmann, Rosina 👤 Kristen-Burmann, Claudia 👤 Bank-Wolf, Barbara 👤 König, Matthias 👤 Herden, Christiane 👤 Hain, Torsten 👤 Thiel, Heinz Jürgen 👤 Ziebuhr, John 👤 Tekes, Gergely ⏰ 2018 📚 mBio

• Vaccines to prevent severe acute respiratory syndrome coronavirus-induced disease

  👤 Enjuanes, Luis 👤 DeDiego, Marta L. 👤 Álvarez, Enrique 👤 Deming, Damon 👤 Sheahan, Tim 👤 Baric, Ralph ⏰ 2008 📚 Virus Research

• An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells

  👤 Fang, Shouguo 👤 Chen, Bo 👤 Tay, Felicia P.L. 👤 Ng, Beng Sern 👤 Liu, Ding Xing ⏰ 2007 📚 Virology

• The nsp3 macrodomain promotes virulence in mice with coronavirus-induced encephalitis

  👤 Fehr, Anthony R. 👤 Athmer, Jeremiah 👤 Channappanavar, Rudragouda 👤 Phillips, Judith M. 👤 Meyerholz, David K. 👤 Perlman, Stanley ⏰ 2015 📚 Journal of Virology

• Molecular determinants of severe acute respiratory syndrome coronavirus pathogenesis and virulence in young and aged mouse models of human disease

  👤 Frieman, Matthew 👤 Yount, Boyd 👤 Agnihothram, Sudhakar 👤 Page, Carly 👤 Donaldson, Eric 👤 Roberts, Anjeanette 👤 Vogel, Leatrice 👤 Woodruff, Becky 👤 Scorpio, Diana 👤 Subbarao, Kanta 👤 Baric, Ralph S. ⏰ 2012 📚 Journal of Virology

• A point mutation within the replicase gene differentially affects coronavirus genome versus minigenome replication

  👤 Galán, Carmen 👤 Enjuanes, Luis 👤 Almazán, Fernando ⏰ 2005 📚 Journal of Virology

• Genetic evidence of a long-range RNA-RNA Interaction between the genomic 5' untranslated region and the nonstructural protein 1 coding region in murine and bovine coronaviruses

  👤 Guan, Bo Jhih 👤 Su, Yu Pin 👤 Wu, Hung Yi 👤 Brian, David A. ⏰ 2012 📚 Journal of Virology

• An optimal cis-replication stem-loop IV in the 5′ untranslated region of the mouse coronavirus genome extends 16 nucleotides into open reading frame 1

  👤 Guan, Bo Jhih 👤 Wu, Hung Yi 👤 Brian, David A. ⏰ 2011 📚 Journal of Virology

• Switching species tropism: An effective way to manipulate the feline coronavirus genome

  👤 Haijema, Bert Jan 👤 Volders, Haukeliene 👤 Rottier, Peter J.M. ⏰ 2003 📚 Journal of Virology

• Live, Attenuated Coronavirus Vaccines through the Directed Deletion of Group-Specific Genes Provide Protection against Feline Infectious Peritonitis

  👤 Haijema, Bert Jan 👤 Volders, Haukeline 👤 Rottier, Peter J.M. ⏰ 2004 📚 Journal of Virology

• The PDZ-Binding Motif of Severe Acute Respiratory Syndrome Coronavirus Envelope Protein Is a Determinant of Viral Pathogenesis

  👤 Jimenez-Guardeño, Jose M. 👤 Nieto-Torres, Jose L. 👤 DeDiego, Marta L. 👤 Regla-Nava, Jose A. 👤 Fernandez-Delgado, Raul 👤 Castaño-Rodriguez, Carlos 👤 Enjuanes, Luis ⏰ 2014 📚 PLoS Pathogens

• Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: Crossing the host cell species barrier

  👤 Kuo, Lili 👤 Godeke, Gert Jan 👤 Raamsman, Martin J.B. 👤 Masters, Paul S. 👤 Rottier, Peter J.M. ⏰ 2000 📚 Journal of Virology

• Structural and functional conservation of cis-acting RNA elements in coronavirus 5'-terminal genome regions

  👤 Madhugiri, Ramakanth 👤 Karl, Nadja 👤 Petersen, Daniel 👤 Lamkiewicz, Kevin 👤 Fricke, Markus 👤 Wend, Ulrike 👤 Scheuer, Robina 👤 Marz, Manja 👤 Ziebuhr, John ⏰ 2018 📚 Virology

• Middle East respiratory syndrome coronavirus nonstructural protein 16 is necessary for interferon resistance and viral pathogenesis

  👤 Menachery, Vineet D. 👤 Gralinski, Lisa E. 👤 Mitchell, Hugh D. 👤 Dinnon, Kenneth H. 👤 Leist, Sarah R. 👤 Yount, Boyd L. 👤 Graham, Rachel L. 👤 McAnarney, Eileen T. 👤 Stratton, Kelly G. 👤 Cockrell, Adam S. 👤 Debbink, Kari 👤 Sims, Amy C. 👤 Waters, Katrina M. 👤 Baric, Ralph S. ⏰ 2017 📚 mSphere

• A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence

  👤 Menachery, Vineet D. 👤 Yount, Boyd L. 👤 Debbink, Kari 👤 Agnihothram, Sudhakar 👤 Gralinski, Lisa E. 👤 Plante, Jessica A. 👤 Graham, Rachel L. 👤 Scobey, Trevor 👤 Ge, Xing Yi 👤 Donaldson, Eric F. 👤 Randell, Scott H. 👤 Lanzavecchia, Antonio 👤 Marasco, Wayne A. 👤 Shi, Zhengli Li 👤 Baric, Ralph S. ⏰ 2015 📚 Nature Medicine

• Murine coronavirus ubiquitin-like domain is important for papain-like protease stability and viral pathogenesis

  👤 Mielech, Anna M. 👤 Deng, Xufang 👤 Chen, Yafang 👤 Kindler, Eveline 👤 Wheeler, Dorthea L. 👤 Mesecar, Andrew D. 👤 Thiel, Volker 👤 Perlman, Stanley 👤 Baker, Susan C. ⏰ 2015 📚 Journal of Virology

• Severe acute respiratory syndrome coronavirus nsp 9 dimerization is essential for efficient viral growth

  👤 Miknis, Zachary J. 👤 Donaldson, Eric F. 👤 Umland, Timothy C. 👤 Rimmer, Ryan A. 👤 Baric, Ralph S. 👤 Schultz, L. Wayne ⏰ 2009 📚 Journal of Virology

• Transgene expression in the genome of middle east respiratory syndrome coronavirus based on a novel reverse genetics system utilizing red-mediated recombination cloning

  👤 Muth, Doreen 👤 Meyer, Benjamin 👤 Niemeyer, Daniela 👤 Schroeder, Simon 👤 Osterrieder, Nikolaus 👤 Müller, Marcel Alexander 👤 Drosten, Christian ⏰ 2017 📚 Journal of General Virology

• SARS coronavirus accessory proteins

  👤 Narayanan, Krishna 👤 Huang, Cheng 👤 Makino, Shinji ⏰ 2008 📚 Virus Research

• Understanding bat SARS-like coronaviruses for the preparation of future coronavirus outbreaks — Implications for coronavirus vaccine development

  👤 Ng, Oi Wing 👤 Tan, Yee Joo ⏰ 2017 📚 Human Vaccines and Immunotherapeutics

• The papain-like protease determines a virulence trait that varies among members of the SARS-coronavirus species

  👤 Niemeyer, Daniela 👤 Mösbauer, Kirstin 👤 Klein, Eva M. 👤 Sieberg, Andrea 👤 Mettelman, Robert C. 👤 Mielech, Anna M. 👤 Dijkman, Ronald 👤 Baker, Susan C. 👤 Drosten, Christian 👤 Müller, Marcel A. ⏰ 2018 📚 PLoS Pathogens

• Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice

  👤 Rockx, Barry 👤 Sheahan, Timothy 👤 Donaldson, Eric 👤 Harkema, Jack 👤 Sims, Amy 👤 Heise, Mark 👤 Pickles, Raymond 👤 Cameron, Mark 👤 Kelvin, David 👤 Baric, Ralph ⏰ 2007 📚 Journal of Virology

• Differential susceptibility of macrophages to serotype II feline coronaviruses correlates with differences in the viral spike protein

  👤 Shirato, Kazuya 👤 Chang, Hui Wen 👤 Rottier, Peter J.M. ⏰ 2018 📚 Virus Research

• Role of phosphorylation clusters in the biology of the coronavirus infectious bronchitis virus nucleocapsid protein

  👤 Spencer, Kelly Anne 👤 Dee, Michael 👤 Britton, Paul 👤 Hiscox, Julian A. ⏰ 2008 📚 Virology

• One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities

  👤 Subissi, Lorenzo 👤 Posthuma, Clara C. 👤 Collet, Axelle 👤 Zevenhoven-Dobbe, Jessika C. 👤 Gorbalenya, Alexander E. 👤 Decroly, Etienne 👤 Snijder, Eric J. 👤 Canard, Bruno 👤 Imbert, Isabelle ⏰ 2014 📚 Proceedings of the National Academy of Sciences of the United States of America

• The Severe Acute Respiratory Syndrome (SARS)-coronavirus 3a protein may function as a modulator of the trafficking properties of the spike protein

  👤 Tan, Yee Joo ⏰ 2005 📚 Virology Journal

• MERS coronavirus nsp1 participates in an efficient propagation through a specific interaction with viral RNA

  👤 Terada, Yutaka 👤 Kawachi, Kengo 👤 Matsuura, Yoshiharu 👤 Kamitani, Wataru ⏰ 2017 📚 Virology

• Establishment of a virulent full-length cDNA clone for type I feline coronavirus strain C3663

  👤 Terada, Yutaka 👤 Kuroda, Yudai 👤 Morikawa, Shigeru 👤 Matsuura, Yoshiharu 👤 Maeda, Ken 👤 Kamitani, Wataru ⏰ 2019 📚 Journal of Virology

• Recombinant live attenuated avian coronavirus vaccines with deletions in the accessory genes 3ab and/or 5ab protect against infectious bronchitis in chickens

  👤 van Beurden, Steven J. 👤 Berends, Alinda J. 👤 Krämer-Kühl, Annika 👤 Spekreijse, Dieuwertje 👤 Chenard, Gilles 👤 Philipp, Hans Christian 👤 Mundt, Egbert 👤 Rottier, Peter J.M. 👤 Verheije, M. Hélène ⏰ 2018 📚 Vaccine

• A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination

  👤 Van Beurden, Steven J. 👤 Berends, Alinda J. 👤 Krämer-Kühl, Annika 👤 Spekreijse, Dieuwertje 👤 Chénard, Gilles 👤 Philipp, Hans Christian 👤 Mundt, Egbert 👤 Rottier, Peter J.M. 👤 Verheije, M. Hélène ⏰ 2017 📚 Virology Journal

• Importance of the penultimate positive charge in mouse hepatitis coronavirus A59 membrane protein

  👤 Verma, Sandhya 👤 Lopez, Lisa A. 👤 Bednar, Valerie 👤 Hogue, Brenda G. ⏰ 2007 📚 Journal of Virology

• Severe acute respiratory syndrome coronavirus evades antiviral signaling: Role of nsp1 and rational design of an attenuated strain

  👤 Wathelet, Marc G. 👤 Orr, Melissa 👤 Frieman, Matthew B. 👤 Baric, Ralph S. ⏰ 2007 📚 Journal of Virology

• Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus

  👤 Weiss, Susan R. 👤 Navas-Martin, Sonia ⏰ 2005 📚 Microbiology and Molecular Biology Reviews

• Proteolytic activation of the porcine epidemic diarrhea coronavirus spike fusion protein by trypsin in cell culture

  👤 Wicht, Oliver 👤 Li, Wentao 👤 Willems, Lione 👤 Meuleman, Tom J. 👤 Wubbolts, Richard W. 👤 van Kuppeveld, Frank J.M. 👤 Rottier, Peter J.M. 👤 Bosch, Berend Jan ⏰ 2014 📚 Journal of Virology

• Genetic manipulation of porcine deltacoronavirus reveals insights into NS6 and NS7 functions: a novel strategy for vaccine design

  👤 Zhang, Mengjia 👤 Li, Wan 👤 Zhou, Peng 👤 Liu, Dejian 👤 Luo, Rui 👤 Jongkaewwattana, Anan 👤 He, Qigai ⏰ 2020 📚 Emerging Microbes and Infections

• Virulence factors in porcine coronaviruses and vaccine design

  👤 Zuñiga, Sonia 👤 Pascual-Iglesias, Alejandro 👤 Sanchez, Carlos M. 👤 Sola, Isabel 👤 Enjuanes, Luis ⏰ 2016 📚 Virus Research

• Sequence Motifs Involved in the Regulation of Discontinuous Coronavirus Subgenomic RNA Synthesis

  👤 Zúñiga, Sonia 👤 Sola, Isabel 👤 Alonso, Sara 👤 Enjuanes, Luis ⏰ 2004 📚 Journal of Virology